Human Genome-wide CRISPRi-v2 Libraries

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Human Genome-wide CRISPRi-v2 Libraries

Human Genome-wide CRISPRi-v2 Libraries

Catalog#： LIBR-H028-P002 LIBR-H028-P100 LIBR-H028-P200 LIBR-H028-P500

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Detailed Product Information Recommended Companion Products
The CRISPR interference library targets 18,905 genes across the human genome and contains a total of 104,535 knockout plasmid vectors, of which 5 different gRNA vectors are designed for each gene, in addition to 1,895 control vectors targeting intergenic sequences. The library uses pCRISPRia-v2 as the backbone, which is a two-plasmid system that expresses only gRNA, while the dCas9-KRAB gene is on a separate vector that needs to be used in conjunction.
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Product Information

Product Name
Human Genome-wide CRISPRi-v2 Libraries
Organism
Human
Library Type
Inhibition Library
Plasmid System
Dual-plasmid System
Virus Packaging System
3rd Lentivirus Packaging System
Targeted Genes
18905
gRNA Number
104535
Non-targeting gRNA Number
1895
Label
Puro, tagBFP
Vector Formula
pCRISPRia-v2 vector formula
Click to view the full image
CRISPR iScreen™ Product Strength
  • 35+ Libraries
    100+ Cas9 cell lines for screening
    35+ Library types in stock, fulfilling different research needs Cas9 cells with high activity, good cell condition, easily accelerate CRISPR library construction.
    1
  • Plasmid
    Coverage>99%, uniformity<10
    The use of self-developed library specific competent cell makes it easier to capture exogenous DNA, with high transformation efficiency and low mutation risk.
    2
  • Cell Pool
    Coverage rate up to 99%
    Exclusive cell pool preparation process can achieve large-scale and standardized production of library cell pool, achieving fewer differences between batches and high repeatability.
    3
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CRISPR Library Screen Service
Provides one-stop solutions for CRISPR-KO, CRISPRa, CRISPRi library screen from high-throughput sgRNA library construction, virus packaging, cell infection, drug screening, NGS sequencing to data analysis, etc. Various deliverables fulfill different research needs.
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Cas9 Stable Cell Line
The Cas9 cell lines in our cell bank can stably express Cas9 protein. So gene knockout can be achieved by transfecting gRNA. Simultaneously transfecting gRNA and donor DNA can achieve gene knock-in/point mutation, effectively improving experimental efficiency.
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